phospho p38mapk antibody  (Proteintech)

Journal: Scientific Reports

Article Title: The hydroxamate based HDAC inhibitor WMJ-J-09 induces colorectal cancer cell death by targeting tubulin and downregulating survivin

doi: 10.1038/s41598-025-04714-w

Figure Lengend Snippet: LKB1-p38 MAPK signaling contributed to WMJ-J-09-mediated p21 and survivin regulation. ( A ) Immunoblot result of p38MAPK phosphorylation in HCT116 cells exposed to WMJ-J-09. ( B , C ) Immunoblot result of p21 ( B ) and survivin ( C ) expression in WMJ-J-09-stimulated HCT116 cells with or without p38 inhibitor III ( D ) Immunoblot result of LKB1 phosphorylation in HCT116 cells exposed to WMJ-J-09 for indicated periods. ( E ) Immunoblot results from the effects of LKB1 siRNA or negative control siRNA on p38MAPK and p53 phosphorylation elicited by WMJ-J-09. ( F ) Immunoblot result of the effects of LKB1 siRNA or negative control siRNA on WMJ-J-09-modulated p21 and survivin expression in HCT116 cells. Each band intensity was quantified, and total α-tubulin levels normalized the fold changes of LKB1, p21, and survivin; total p53 levels normalized that of p53 phosphorylation; total p38MAPK levels normalized that of p38MAPK phosphorylation; total LKB1 levels normalized that of LKB1 phosphorylation. Error bars, mean ± S.E.M. (shown only for independent replicate experiments with n ≥ 4). One-way ANOVA followed by Tukey’s post-hoc test assessed statistical significance (*p < 0.05 compared to the control group).

Article Snippet: Antibodies against α-tubulin Lys40 acetylation, p53, p53 Lys379 acetylation, p53 Ser15 phosphorylation, p38MAPK Thr180/Tyr182 phosphorylation, p38MAPK, LKB1, LKB1 Ser428 phosphorylation, PARP, cleaved caspase 3 (active form) and survivin were from Cell Signaling (Danvers, MA, U.S.A.).

Techniques: Western Blot, Phospho-proteomics, Expressing, Negative Control, Control

Journal: Pharmaceutical Biology

Article Title: Integrated pharmacoanalysis, bioinformatics analysis, and experimental validation to identify the ingredients and mechanisms of Xiao-Luo-Wan in uterine fibroids treatment

doi: 10.1080/13880209.2025.2485905

Figure Lengend Snippet: Molecular docking models of XLW components that bind to potential targets in 3-dimensional stereoimages. Molecular docking pattern diagrams of (A) TRIM9 and buergerinin B, (B) TRIM9 and cedrol, (C) TRIM9 and ent-15B-16-epoxy-kauan-17-ol, (D) NF-κB p65 and buergerinin B, (E) NF-κB p65 and cedrol, (F) NF-κB p65 and ent-15B-16-epoxy-kauan-17-ol, (G) p38MAPK and buergerinin B, (H) p38MAPK and cedrol, and (I) p38MAPK and ent-15B-epoxy-kauan-17-ol.

Article Snippet: Next, the membranes were blocked with Tris-buffered saline containing 5% nonfat milk for 1 h and incubated with primary antibodies against Ki-67 (1:5000; Abcam, Cat#: 92742, RRID:AB_10562976), Caspase9 (1:1000; CST, Cat#: 9508, RRID:AB_2068620), TRIM9 (1:1000; CST, Cat#: 47990, RRID: AB_3668999), NF-κB p65 (1:1000; CST, Cat#: 8242, RRID:AB_10859369), p-p38MAPK (1:1000; CST, Cat#: 9211, RRID:AB_331641) and β-actin (1:1000; CST, Cat#: 4967, RRID:AB_330288) overnight at 4 °C.

Techniques:

Journal: Pharmaceutical Biology

Article Title: Integrated pharmacoanalysis, bioinformatics analysis, and experimental validation to identify the ingredients and mechanisms of Xiao-Luo-Wan in uterine fibroids treatment

doi: 10.1080/13880209.2025.2485905

Figure Lengend Snippet: The 3 components with the highest affinity for candidate protein targets.

Article Snippet: Next, the membranes were blocked with Tris-buffered saline containing 5% nonfat milk for 1 h and incubated with primary antibodies against Ki-67 (1:5000; Abcam, Cat#: 92742, RRID:AB_10562976), Caspase9 (1:1000; CST, Cat#: 9508, RRID:AB_2068620), TRIM9 (1:1000; CST, Cat#: 47990, RRID: AB_3668999), NF-κB p65 (1:1000; CST, Cat#: 8242, RRID:AB_10859369), p-p38MAPK (1:1000; CST, Cat#: 9211, RRID:AB_331641) and β-actin (1:1000; CST, Cat#: 4967, RRID:AB_330288) overnight at 4 °C.

Techniques:

Journal: Pharmaceutical Biology

Article Title: Integrated pharmacoanalysis, bioinformatics analysis, and experimental validation to identify the ingredients and mechanisms of Xiao-Luo-Wan in uterine fibroids treatment

doi: 10.1080/13880209.2025.2485905

Figure Lengend Snippet: Effects of XLW decoction-treated serum on the (A) mRNA and (B) protein expression of TRIM9, NF-κB and p-p38MAPK in UMCs. * p < 0.05 compared with the control. # p < 0.05 compared with RU-486. RU-486: mifepristone; XLW-L: 20% XLW drug-containing serum; XLW-M: 30% XLW drug-containing serum; XLW-H: 40% XLW drug-containing serum.

Article Snippet: Next, the membranes were blocked with Tris-buffered saline containing 5% nonfat milk for 1 h and incubated with primary antibodies against Ki-67 (1:5000; Abcam, Cat#: 92742, RRID:AB_10562976), Caspase9 (1:1000; CST, Cat#: 9508, RRID:AB_2068620), TRIM9 (1:1000; CST, Cat#: 47990, RRID: AB_3668999), NF-κB p65 (1:1000; CST, Cat#: 8242, RRID:AB_10859369), p-p38MAPK (1:1000; CST, Cat#: 9211, RRID:AB_331641) and β-actin (1:1000; CST, Cat#: 4967, RRID:AB_330288) overnight at 4 °C.

Techniques: Expressing, Control

Journal: Nutrition Research and Practice

Article Title: The edible ethanol extract of Rosa hybrida suppresses colon cancer progression by inhibiting the proliferation-cell signaling-metastasis axis

doi: 10.4162/nrp.2025.19.1.14

Figure Lengend Snippet: EERH, ethanol extract of Rosa hybrida ; ERK, extracellular signal-regulated kinases1/2; JNK, c-Jun N-terminal kinases; AKT, protein kinase B; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; p38 MAPK, p38 mitogen-activated protein kinases; HCT116, human colorectal carcinoma cell line; U0126, MEK inhibitor; SP600125, JNK inhibitor; LY294002, AKT inhibitor. * P < 0.05; # P < 0.05.

Article Snippet: Antibodies against p38MAPK (#9212), phospho-p38MAPK (#9211), c-Jun NH2-terminal kinase (JNK; #9258), phospho-JNK (#9251), extracellular signal-regulated kinases1/2 (ERK; #9102), phospho-ERK (#9101), AKT (#9272), phospho-AKT (#9271), normal rabbit immunoglobulin G (IgG; #2729S), and mouse IgG Isotype control (#5415S) were acquired from Cell Signaling (Danvers, MA, USA).

Techniques: